How to Identify the Slides:
Unstained & special stained sections:
- Ground compact bone: unstained section with Haversian canal
- Nerve trunk (osmic acid): black circles of myelin sheath
- Spinal ganglion (Ag): brown nerve cells arranged in group.
- Sympathetic ganglion (Ag): brown scattered small nerve cells
Large hollow sections:
- Aorta: Thick wall with elastic membranes.
- Decalcified compact bone: Haversian systems OR central BM cavity
Small hollow sections:
- Basilar artey: Prominent thick internal elastic lamina.
- Medium sized A & V: The artery… Thick wall with narrow lumen
The vein… Thin wall with wide lumen
- T.S skeletal muscle: Acidophilic cytoplasm with periphral nuclei.
- Thymus: lobules with outer dark cortex & inner pale medulla
Sections with lymphatic follicles:
-Lymph Node: cortical follicles & medullary cord.
- Spleen: Scattered lymph follicles with central arteriole
- Tonsil: St. sq.epith. forming crypts + lymphatic follicles + Mucous glands.
Dot shaped slides:
- Nerve trunk (H&E): the bundles of nerve fibers
- Spinal ganglion (H&E): rounded nerve cells in groups or rows.
- Moderator band:Large purkinje fibers with peripheral myofibrils.
Eye glasses sections:
- Costal cartilage: basophilic matrix & chondrocytes in their lacunae.
- Spongy bone: multiple irregular bone marrow cavities
Chracteristically shaped sections;
- Growing bone: presence of cartilage then replaced by bone.
- Umbilical cord: mesenchymal cells are stellate OR pale matrix.
- Ear pinna: Cartilage with hairy skin on both sides
-L.S skeletal muscle: parallel cylindrical muscle fibers OR
multiple peripheral nuclei.
- Cardiac muscle and valve: muscle fibers in different directions
& presence of the valve.